List of Figures

A. Localization of Molecular Chaperones in Rat Liver Hepatocytes

  1. Western immunoblotting analysis of homogenates from rat liver and C. vinosum
  2. Thin sections of C. vinosum, fixed with 1% glutaraldehyde, embedded in Lowicryl K4M and labeled by the protein A-gold

    technique

    Quantitative analysis of the distribution of the gold particles for both proteins, the cpn10 and cpn60

  3. Paraffin sections of rat liver tissue processed by immunofluorescence to demonstrate the distribution of cpn10 and cpn60

  4. Immunocytochemical detection of cpn10 antigenic sites on rat liver tissue using the protein A-gold technique

  5. Immunocytochemical detection of cpn60 antigenic sites on rat liver tissue using the protein A-gold technique

  6. Quantitative analysis of the distribution of the gold particles revealing the cpn10 and the cpn60 antigenic sites in mitochondria and peroxisomes of rat hepatocytes

 

B. Localization of Molecular Chaperones in Rat Pancreatic Tissue

  1. Western immunoblotting analysis of rat pancreas homogenate
  2. Paraffin sections of rat pancreatic tissue processed by immunofluorescence
  3. Immunocytochemical detection of cpn10 antigenic sites on rat pancreatic tissue
  4. Immunocytochemical detection of cpn60 antigenic sites on rat pancreatic tissue
  5. Immunocytochemical detection of cpn10 in the mouse pancreatic duct using the protein A-gold technique. Inset: Western immunoblotting analysis of the mouse pancreatic juice
  6. Immunocytochemical detection of cpn10 antigenic sites on guinea pig (A) and human (B) pancreatic acinar cells using the protein A-gold technique
  7. Immunocytochemical detection of hsp70 antigenic sites on rat pancreatic tissue
  8. Double labeled pancreatic acinar cells for acid phosphatase activity and hsp70 immunoreactive sites

 

C. Molecular Chaperones in RER Intracisternal Crystals

  1. Electron micrograph showing features of pancreatic acinar cells displaying CPME-induced crystals
  2. Protein A-gold immunocytochemical detection of amylase by pancreatic acinar cells displaying CPME-induced crystals
  3. Protein A-gold immunocytochemical detection of cpn10 antigenic sites on pancretic tissue from CPME-treated rat
  4. Protein A-gold immunocytochemical detection of cpn60 antigenic sites on pancreatic tissue from CPME-treated rat
  5. Protein A-gold immunocytochemical detection of hsp70 antigenic sites on pancreatic tissue from CPME-treated rat
  6. Quantitative evaluation of labeling densities for chaperones in acinar cells from normal (A) and CPME-treated (B) rats

 

 

List of Tables

 

A. Rat liver hepatocytes

  1.  Quantitative evaluation of cpn10 and cpn60 immunolabeling in rat hepatocyte

 

B. Rat pancreatic acinar cells

  1. Densities of labeling obtained with the anti-cpn10, anti-cpn60 and anti-hsp70 over different cellular compartments of the rat pancreatic acinar cells
  2. Quantitative immunocytochemical localization of cpn10, cpn60 and hsp70 under specific and control conditions

 

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